Fsc-a !!exclusive!!

When a cell passes through a focused laser beam, it scatters light in various directions.

Run a mix of small (3µm) and large (6-10µm) beads to check the dynamic range. Adjust FSC voltage so both populations are on scale (usually between 10^2 and 10^5 on a log scale or 100-200K on a linear scale). When a cell passes through a focused laser

If you have spent any time at the flow cytometer, you know the first button you hit is (Forward Scatter). It tells you about cell size. But what about FSC-A ? Often displayed right next to FSC-H (Height) or FSC-W (Width), this parameter is the unsung hero of data quality. When a cell passes through a focused laser